- ￥4978
- 赛默飞（Thermo Fisher）
- PA5102429
- 2025年07月12日
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文献和实验Use of RNA Interference to Investigate Cytokine Signal Transduction in Pancreatic Beta Cells
to the cytokines interleukin(IL)-1β + interferon(IFN)-γ or to tumor necrosis factor(TNF)-α + IFN-γ induces beta cell dysfunction and ultimately apoptosis. The transcription factors NF-κB and STAT1 are key regulators of cytokine-induced beta cell death
tumor necrosis factor–{alpha} (TNF-{alpha}), interferon-beta (IFN-beta), and IFN-{gamma} (1). The authors observed that of ~200 miRNAs examined, only miR-155 increased in amount in response to both TLR ligands and IFN-beta. miR-155 accumulated
, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v
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