- ￥4428
- 赛默飞（Thermo Fisher）
- PA5101039
- 2025年07月09日
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文献和实验. Here, we describe the detection of autoAbs to various self-antigens with different tissue restriction patterns which includes collagens, k-α1 tubulin, vimentin, and myosin. We also discuss their relevance in monitoring for rejection following solid organ transplantation.
Preparation of Pure Tyrosinated or Detyrosinated Tubulin Isoforms
Alpha tubulin comprises a C-terminal tyrosine residue, which is subject to cyclic removal from the peptide chain by a still uncharacterized carboxypeptidase and re-addition to the chain by a tubulin tyrosine ligase. We have shown in different
Labeling Tubulin and Quantifying Labeling Stoichiometry
polymerization. 2. Layer polymerized tubulin onto 20 ml warm High pH Cushion in two 50.2 Ti tubes. Pellet microtubules in a Beckman ultracentrifuge in a 50.2 Ti rotor at 40K for 45' at 35¡C. 3. Aspirate the supernatant above the cushion and rinse
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