- ￥6611
- 赛默飞（Thermo Fisher）
- PA185871
- 2025年07月16日
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- 规格：
ANTIGEN RABBIT POLYCLONAL
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文献和实验make an Antibody against a Peptides
to create an immunogen. One-step chemical reactions are easy with Pierce Sulfo-SMCC Pre-Activated Carrier Proteins (Figure 1). If your hapten does not have a sulfhydryl group, you can use EDC chemistry (Figure 2) to couple amines (lysine) or carboxylic acids
, bleeding procedure, obtaining serum, precipitation of IgG. ・ GST-fusion protein injection into rabbits (IVAN J. DELGADO ORLIC) ・ How Do I make an Antibody against a Peptides (Pierce) ・ A fast and inexpensive method
, 5% BSA, pH 7.4) for 2 h at room temperature and then probed for CTGF by incubation for 40 min with anti-CTGF antibody at 0.5 µg/ml in blocking buffer. After extensive washing at 37°C, the filters were incubated with either HRP-conjugated donkey anti
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