- ￥5134
- 赛默飞（Thermo Fisher）
- PA146160
- 2025年07月12日
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文献和实验information about events occurring in vivo, and many of the early tissue culture studies were carried out to enable direct observations of living cells Fig. 1. Movements and changes in shape of the growing tip of a frog embryo nerve fiber
, 2.47g All the components except for MnCl2 were mixed and the pH was adjusted to 6.7 with 0.1 N HCl (If you overshoot, do not readjust the pH by adding base, instead, discard the solution and begin again). Then 2.475 g MnCl2 was added to the solution
to a final concentration of 0.1 mg/ml, and incubate at room temperature for 15-30 minutes. 8)Using 4 Qiagen-tip 500, pre-purify the supernatant as instructed by the Qiagen procedure. Qiagen tips are pre-equilibrated with QBT, then the supernatant is applied
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