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文献和实验Protein purification: skeletal muscle myosin
Overview SKELETAL MUSCLE MYOSIN A two-headed hexomeric actin-activated ATPase consisting of two heavy chains of ~200 kDa and four light chains of ~20kDa. The tail portion
Isolation of Actin and Myosin Filaments
0.05 M Sodium phosphate buffer, pH 7.0 with 0.001 M EDTA. Blend this mix in a standard blender at low speed and pour the slurry into a large beaker. Upon settling, the muscle fragments will settle on top of the underlying connective
Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
Cultured embryonic and adult skeletal muscle cells have a number of different uses. The microdissected explant technique described in this chapter is a robust and reliable method for isolating relatively large numbers of proliferative
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