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PBS ONLY 1 mg
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文献和实验Phosphoamino acid analysis:Mark Kamps's method
more than 3 µl is tedious,so don't use much H20.On the other hand,I wouldn't use much less than 6 µl so as to have good recovery. 7.Spot 1 µl PAA standards (about 0.3 µg each of PSer,PThr,and PTyr)on a cellulose thin layer chromatography plate and then spot
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
phosphorylation. This unit describes the use of phospho?motif antibodies to elucidate the kinase(s) responsible for phosphorylating substrate proteins. Curr. Protoc. Mol. Biol. 101:18.20.1?18.20.11. © 2013 by John Wiley & Sons, Inc. Keywords
多核苷酸磷酸化酶polynucleotide phospho-rylase
S. Ochoa、 M. Grunberg- Monago( 1955),在棕色固氮菌( Azotobacter vinelandii)中发现的酶,广泛存在于微生物中。催化( NMP) 因为对核苷酸的特异性低,可以用来合成各种多聚物。也可以由大肠杆菌、藤黄微球菌( Micro- coccus luteus)精制出高纯度的酶,分子量均约 20万,性质也相同。聚合反应时有作为引物( primer)的寡核苷酸起作用,对于反应速度几乎没有影响。另一方面,逆反应是从
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