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文献和实验Biosensors for Characterizing the Dynamics of Rho Family GTPases in Living Cells
in which an environmentally sensitive dye is used to report the activation of endogenous Cdc42, followed by two biosensors based on FRET, one using intramolecular and the other intermolecular FRET. The design differences lead to the need for different approaches in imaging
V294.PART II,Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
has been cleaned use a 19-gage needle to pierce both ends of thebone. Keep the needle in one end of the bone (fatter end). Using a 5-mL syringe,flush the bone marrow out of the bone with 5 mL of macrophage starve mediuminto a 15-mL Falcon tube. Add an additional
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