- ￥6057
- 赛默飞（Thermo Fisher）
- OSS00051G-500UG
- 2025年07月13日
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文献和实验Site‐Specific Protein Labeling with SNAP‐Tags
2 ; e.g., Invitrogen) Non‐fluorescent SNAP‐cell blocking reagents: SNAP Cell Block (NEB, cat. no. S9106, cell permeable), SNAP‐Surface Block (NEB, cat. no. S9143, cell impermeable), BG‐NH
RUVKUN Antibody Staining Protocol revised 10/29/90
RUVKUN Antibody Staining Protocol revised 10/29/90 Fixation: 1. C. elegans N2 grown in liquid or plates. Harvest worms. Wash for 2 hours at room temp. in 1X M9 or PBS. 2. Fix in 5 ml of FRESH THAT DAY 1% paraformaldehyde:
off with 0.05% Tween 20 in PBS. 7. Block for about 1 hr with 5% BSA/Tween 20, or overnight with 3% BSA/Tween 20. Reagents and Buffers for Westerns Reagent 30% acrylamide/bisacrylamide (29:1 mix) 100ml(dark bottle at 4°C) 29g acrlamid 1g bisacrylamid
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