- ￥4665
- 赛默飞（Thermo Fisher）
- OSR00263W-100UL
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 规格：
100 UL
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文献和实验E.Z.N.A. Mag-Bind Blood DNA Protocol (1-100 ul Blood)
tubes or microplates 实验步骤 1. Add 1-100 ul blood sample to a nuclease-free microcentrifuge tube. Bring the volume up to 100 ul with Elution Buffer provided with this kit. 2. Add 100 ul Buffer MSL and 10 ul Proteinase K
S1 Nuclease Protection Mapping
The use of S1 nuclease to map the start site of a transcription unit is a well-established technique. Based on the method of Berk and Sharp (1 ), it has undergone many refinements over the years. S1 nuclease mapping requires a relatively
4. Vortex vigorously. 5. Incubate at 100 deg C for 3 minutes. 6. Transfer immediately to 58 deg C and incubate overnight. S1 digestion buffer (final concentration): 300 mM NaCl 30 mM sodium acetate, pH 5.5 2 mM zinc sulfate 1000 units/ml S1 nuclease
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