- ￥4665
- 赛默飞（Thermo Fisher）
- OSM00140W-100UL
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 规格：
100 UL
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文献和实验E.Z.N.A. Mag-Bind Blood DNA Protocol (1-100 ul Blood)
tubes or microplates 实验步骤 1. Add 1-100 ul blood sample to a nuclease-free microcentrifuge tube. Bring the volume up to 100 ul with Elution Buffer provided with this kit. 2. Add 100 ul Buffer MSL and 10 ul Proteinase K
Factor Xa Cleavage of MBP-Fusion protein
2. Optimum pH 6.5. Phosphate buffer reduce activity. Use TrisHCl or HEPES. Detergents: Up to 1% Triton X-100, NP40 or n-octylglucoside. Reductants: DTT not recommended. ß-Mercaptoethanol up to 5mM NaCl: Decreasing activity at > 100mM NaCl. Imidazol
Purification of MBP (maLTose-binding proteins) Fused Proteins
. Resuspend pellet in 100 ml buffer for analysis on SDS-PAGE. Elute "unbound" proteins from amylose resin by washing resin with 10 column volumes of 20 mM Tris pH7.4, 200 mM NaCl, 10mM b ME, 1 mM EDTA. Elute bound MBP fusion protein
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