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文献和实验Histone Acetylation and Deacetylation
transcription (1 ,2 ). DNA is packaged into chromatin, a highly organized and dynamic protein-DNA complex. The fundamental subunit of chromatin, the nucleosome, is composed of an octomer of four core histones, an H3/H4 tetramer and two H2A/H2B dimers, surrounded
as reference: a standard gel should give about 10ul per core. 6. Stain remainder of gel, view and photograph at 254nm to ensure correct regions were sampled. NOTE: IT IS POSSIBLE TO QUICKLY CORE A STAINED GEL DIRECTLY ON A 305 OR EVEN A 254 NM UV BOX
Transgenic Mouse Core Facility
pellet in 100ul 10mM Tris, 0.1mM EDTA. Put through a millipore basket filter as described above. 4.Quantitate/Validate DNA Make a 1:50 dilution and read A260/A280 on spectrophotometer with the microcuvette. (Pure DNA A260/A280=1.8) Cut 1 ug insert
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