SODIUM/HYDROGEN EXCHANGER 1 AN

The Sodium-Hydrogen Exchange System in the Heart: A Target for the Protection of the Ischemic and Reperfused Heart and Inhibition of Heart Failure

Sodium-hydrogen exchange (NHE) is among the most important processes involved in pH regulation in the cardiac cell, especially under ischemic conditions. There is now excellent evidence that stimulation of NHE contributes to paradoxical induction

Porcine brain tubulin preparation

实验试剂   100g P-11 cellulose phosphate fibrous cation exchanger (Whatman Inc, Clifton NJ) 6L 0.1M HCl 6L 0.1 M NaOH 2L 0.1 M MgSO4 2L 10x Column Buffer ~10 L 1x column buffer 10 M NaOH 300 ml

Preparation of tubulin

I have done.  The protocol calls for 3 pig brains, and should yield ~ 60 mg of purified tubulin. Solutions and reagents: 100g P-11 cellulose phosphate fibrous cation exchanger (Whatman Inc, Clifton NJ) 6L  0.1M HCl 6L  0.1 M NaOH 2L 0.1 M MgSO4 2L 10