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文献和实验Cell Surface Immunofluorescence Staining Protocol
-AAD with either PE-Cy5 or PE-Cy7 antibody conjugates. 4) Incubate on ice for 3-5 minutes in the dark. 5) Analyze with a Flow Cytometer. 5. Immunofluorescent Staining of Whole Blood: 1) Add predetermined optimum concentrations
Protocol for intracytoplasmic staining of cytokines for FACS analysis
+ 1% BSA, + 5% heat inactivated normal rabbit serum (HINRS). 9) Label with antibodies to surface molecules that are resistant to fixation (30 mins 4 deg C) eg. anti-CD4 (H129.19-Quantum Red; Sigma R 3637) for FL3 anti-CD44 (Pgp-1:IM
Assay Cell Enrichment and Detection Kit(PE)Mouse IL-2 Secretion Assay Cell Enrichment and Detection Kit(PE)Mouse IL-4 Secretion Assay Cell Enrichment and Detection Kit(PE) Mouse IL-5 Secretion Assay Cell Enrichment and Detection Kit(PE) Mouse IL-10
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