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文献和实验Characterization of Melanocortin Receptors
CHO‐K1 cells expressing a recombinant melanocortin receptor (MC3R, MC4R and MC5R membranes are available from Perkin Elmer Life Sciences) Culture medium (see recipe
like primary osteoblasts or human osteoprogenitor cell line hFOB 1.19 along with murine preosteoblast cell line MC3T3 E-1. Our results showed increase in Rspo 1 at transcript level during differentiating phase of human primary osteoblasts and human FOB 1.19
secondary antibody review -- data from 99 publications
Laboratories 15 AP western blot 15 rhodamine immunocytochemistry 1:500 15 FITC immunocytochemistry 16 sheep Alexa Fluor 568 immunocytochemistry 1:250 Invitrogen 16 streptavidin-horseradish peroxidase
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