- ￥5999
- 赛默飞（Thermo Fisher）
- MA554759
- 2025年07月12日
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文献和实验Isolation of Recombinant MVA Using F13L Selection
. This chapter describes the use of an efficient and easy-to-use selection system adapted for MVA. The system is based on the requirement of the viral gene F13L for efficient virus spread in cell culture, which results in a severe block in virus transmission
Virus Infection of Fibroblasts
containing 10% calf serum (prepare dilutions just before use): a. 1:10 (150 μl virus stock + 1,350 μl media) b. 1:100 (150 μl of 1:10 virus stock + 1,350 μl media) c. 1:1,000 (150 μl of 1:100 virus stock + 1,350 μl media) d. 1:10,000 (150 μl
applications. 10. For 12-well plate: (if in 6-well plate, duplicate all) Infect mouse cells: 400-800 μl virus + 800-400 μl fresh medium =1.2 ml total Infect human cells: 100-500 μl virus + 1100 μl-700 μl 11. Add 8-10 μg/ml polybrene, spin for 30 min
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