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文献和实验破伤风梭菌( clostridium tetani )是引导起破伤风的病原菌,大量存在于人和动物肠道中,由粪便污染土壤,经伤口感染引起疾病。 破伤风梭菌菌体细长,长 4 ~ 8um ,宽 0.3 ~ 0.5um ,周身鞭毛,芽胞呈圆形,位于菌体顶端,直径比菌体宽大,似鼓槌状,是本菌形态上的特征。繁殖体为革兰氏阳性,带上芽胞的菌体易转为革兰氏阴性。破伤风梭菌为专性厌氧菌,最适生长温度为 37 ℃ ph7.0 ~ 7.5 ,营养要求
In Vitro Transcription and Translation in a Cell-Free System from Clostridium tetani
transcription/translation system generated from a nontoxigenic strain of Clostridium tetani (7 ). The cell-free system is prepared in analogy to the Zubay system from E. coli (8 ) with modifications. The cells are lysed with a French press and centrifuged
ClosTron-Mediated Engineering of Clostridium
, Clostridium tetani , and Clostridium perfringens ), the vast majority of the genus are entirely benign, and are able to undertake all manner of useful biotransformations. Prominent amongst them are those species able to produce the biofuels, butanol
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