- ￥3485
- 赛默飞（Thermo Fisher）
- MA554236
- 2025年07月15日
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100
- 规格：
100 UG
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文献和实验Protein Expression and Purification of RPA70-AB (181-422)
),and a gradient program running for 2 column size using Buffer A and Buffer B (same as Buffer A but containing 0.3 M Imidazole).13.Pool fractions after checking them on SDS-PAGE.TEV Protease CleavageComment: TEV protease has a 6x His-tag on its NT
Genotyping using Affymetrix arrays
adaptor (5’-ATT ATG AGC ACG ACA GAC GCC TGA TCT-3’ and 5’phosphate AGC TAG ATC AGG CGT CTG TCG TGC TCA TAA-3’) (Affymetrix) are ligated to the digested DNAs with T4 DNA Ligase (New England BioLabs) in 25µL for 2 hours at 16°C. The ligations are stopped
Geno typing using Affymetrix arrays
, 0.25 µM of XbaI adaptor (5’-ATT ATG AGC ACG ACA GAC GCC TGA TCT-3’ and 5’phosphate ?CTA GAG ATC AGG CGT CTG TCG TGC TCA TAA-3’)(Affymetrix), or HindIII adaptor (5’-ATT ATG AGC ACG ACA GAC GCC TGA TCT-3’ and 5’phosphate ?AGC TAG ATC AGG CGT CTG TCG TGC
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