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文献和实验Western Blotting with Alkaline Phosp
Western Blotting with Alkaline Phosphatase ConjugatesSAMPLE PREPARATIONFor Protein Concentration Determination of Cell Culture1.Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline (PBS).2.Aspirate
Alkaline Phosphatase (EC 3.1.3.1)
bacteria, fungi, invertebrates, fish, and mammals (being located in many organs, including bone marrow, kidney, placenta, and intestinal mucosa), but have not been isolated from higher plants. The most commonly used alkaline phosphatases (AP
The single-cell gel electrophoresis or comet assay was initially reported in 1984 as a novel technique to directly visualize DNA damage in individual cells (1 ). The method was modified by adding strong alkaline conditions to denature genomic
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