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文献和实验in avidity provided by the multimerization of Fc regions, combined with robust detection methods based on numerous commercially available secondary reagents directed against the Fc tag. We describe a set of methods for subcloning, expression, and purification
Chromatin Interaction Analysis Using Paired‐End Tag Sequencing
Analysis using Paired?End Tag sequencing (ChIA?PET) is a technique developed for large?scale, de novo analysis of higher?order chromatin structures. Cells are treated with formaldehyde to cross?link chromatin interactions, DNA segments bound by protein
Gene Activation by T-DNA Tagging
Agrobacterium -mediated transformation of plants has not only become a useful tool for the introduction of chimeric genes into plants, but it has also recently been utilized in gene tagging (for reviews, see refs. 1 –3 ). T-DNA tagging
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