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文献和实验. Here, we describe the detection of autoAbs to various self-antigens with different tissue restriction patterns which includes collagens, k-α1 tubulin, vimentin, and myosin. We also discuss their relevance in monitoring for rejection following solid organ transplantation.
Preparation of Pure Tyrosinated or Detyrosinated Tubulin Isoforms
Alpha tubulin comprises a C-terminal tyrosine residue, which is subject to cyclic removal from the peptide chain by a still uncharacterized carboxypeptidase and re-addition to the chain by a tubulin tyrosine ligase. We have shown in different
Sporulation and Dissection of Yeast a/alpha Diploids
Sporulation and Dissection of Yeast a/alpha Diploids Linda Hoskins Last Modified Tue, Nov 6, 2001 These conditions work well for the Hieter yeast strains 1. Isolate diploid colonies. Inoculate 2 ml YPD + Ade with a single
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