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文献和实验The Polymerase Chain Reaction (PCR)
Topics • What is the Polymerase Chain Reaction? • History and (pre-history) of PCR • How PCR works • Optimising PCR • Fidelity, errors and cloning • PCR primer design • Applications of PCR What is the Polymerase Chain Reaction? • It’s
J chain|J 链[见于IgA分子,与J基因区段无关] jacobine|千言光碱 jasmonic|茉莉酸 jejunum|空肠 jenseniin|詹氏(丙酸)杆菌素 joining gene|J基因[为连接区(J区)编码的基因] joining region|连接区,J区[位于免疫球蛋白等分子的V区与C区之间] josamycin|交沙霉素 juglone|胡桃醌,5-羟-1,4-萘醌 jumping library|跳查文库 junk DNA|无用DNA,无功能DNA[基因
Myosin Light Chain Preparation
in 30 second intervals with 30 second rests until total blend time is 90 seconds. 4.The homogenate is spun at 4200 rpm for 15 minutes in the Beckman J-6 using 1 liter bottles. 5.Discard the supernatant and place the pellets on ice.Add homogenizing
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