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文献和实验Alphaviruses remain important emerging mosquito-borne, zoonotic pathogens that cause both localized human outbreaks and epizootics (e.g., Venezuelan equine encephalitis) and large human epidemics (e.g., Chikungunya). Alphaviruses are globally
Alphavirus vectors have been developed for transient gene expression. The most common approach has been to generate replication-deficient vectors based on Semliki Forest virus, Sindbis virus, or Venezuelan equine encephalitis virus
Sindbis Virus-Based Vectors for the Study of Class I Antigen Presentation In Vitro and In Vivo
, and ability to express large amounts of protein (13 ). Several members of the Alphavirus genus, including Sindbis virus (SIN), Semliki Forest virus, and Venezuelan equine encephalitis virus, have been modified genetically for use as viral expression vectors
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