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文献和实验Detection of Class Switching and IgE Synthesis in Human B Cells
events leading to IgE synthesis. This chapter will illustrate how human B cells (na�ve or memory) can be purified, stored, and cultivated in medium that is permissive for IgE synthesis and stimulated with IL-4 or IL-13 and CD40 activation
Detection of Known Allergen-Specific IgE Antibodies by Immunological Methods
are incubated with the respective diluted serum sample (approximately 25 μL serum required) and bound IgE antibodies are detected with a secondary horseradish peroxidase (HRP) labelled anti-human-IgE antibody via chemiluminescence. The measurement
Mapping of Human T-Cell Epitopes of Allergens
in the process of allergic sensitization, the generation of specific T-helper type 2 (Th2) lymphocytes, which play a crucial role in stimulating B lymphocytes to produce allergen-specific IgE. T-helper (Th) cells play a key role in the regulation
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