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文献和实验Alkaline Phosphatase (EC 3.1.3.1)
bacteria, fungi, invertebrates, fish, and mammals (being located in many organs, including bone marrow, kidney, placenta, and intestinal mucosa), but have not been isolated from higher plants. The most commonly used alkaline phosphatases (AP
The single-cell gel electrophoresis or comet assay was initially reported in 1984 as a novel technique to directly visualize DNA damage in individual cells (1 ). The method was modified by adding strong alkaline conditions to denature genomic
. The idea behind using the same restriction enzymes is to create complementary sticky ends, which will facilitate ligation later on. A phosphatase (commonly Calf Intestinal Alkaline Phosphatase; CIAP) is also added to prevent self-ligation of the destination
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