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文献和实验Method for Purification of Human Hematopoietic Stem Cells by Flow Cytometry
in Table 1 . Among them, CD34 has become the most critical cell-surface marker for positively selecting a rare cell population (1 ,2 ). Within the CD34+ cell population, the differential expression of Thy-1, CD38, and AC133 have been used to fractionate
Generation of Dendritic Cells from Lymphoid Precursors
(9 ). The method described here consists of preparing mononuclear cells (MNC) from BM that are depleted by negative selection of cells expressing lineage markers (CD2, CD3, CD19, CD20, CD14, CD15, CD32, glycophorin A), found on T cells, B cells, monocytes
Native Chromatin Preparation and Illumina/Solexa Library Construction
19, CD36, CD56, CD123, and glycophorin A. 9. Mix well and incubate for 10 min at 4°C-8°C or on ice. 10. Add 30 µL of T cell isolation buffer per 107 cells. 11. Add 20 µL of anti-biotin microbeads (from T
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