- ￥6064
- 赛默飞（Thermo Fisher）
- MA548033
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 规格：
200 UG
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文献和实验1X SDS running buffer.4. Run gel at 25 mA (constant) for 30 min to 1 hr. (or until blue dye just runs off of the gel)5. Transfer onto immobilon P membrane using the SD semi-dry transfer apparatus, per apparatus instructions.6. After transfer, ensure
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
they were raised against and to determine the sensitivity of the antibody for its antigen. Protein samples are prepared with SDS and run non-reduced and reduced on an appropriate SDS-PAGE gel. The proteins are transferred to a PVDF membrane using a semi-dry
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