- ￥6064
- 赛默飞（Thermo Fisher）
- MA547990
- 2025年07月10日
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200 UG
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文献和实验antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
In situ hybridisation to alpha satellite sequences (chromosome specific)
solution and replace with mouse anti-biotin antibody diluted 1:100 in blocking solution for 40 minutes at 37C. We currently use DAKO antibody #M0743, 100-200m l per slide. (10) Wash with blocking solution for 10 minutes (11) Incubate with rabbit
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
consists of 5% (w/v) dried skim milk in Trisbufferedsaline/Tween-20 (TBS-T) buffer (50 mM Tris-HCl,pH 7.5, 150 mM NaCl, and 0.05% (v/v) Tween-20). 4. All antibodies are diluted 1:1,000 in 1% (w/v) dried skim milkin TBS-T, except the CRMP2 antibody (1:250
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