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文献和实验Monoclonal Antibody Production Protocol
for 5 - 10 min. Aspirate the medium, resuspend pellet and wash again with 30ml of IMDM. One immunized spleen has approx. 108 cells. After this wash the cells are ready for the fusion. Myeloma cell preparation: It is essential that the myelomas be free
secondary antibody review -- data from 99 publications
localization of tagged SAX-3 in 293T cell Molecular Probes 2 Alexa Fluor 647 immunocytochemistry localization of BCG phagosomes 3 Alexa Fluor 647 immunohistochemistry detect surface DFz2 3 FITC immunohistochemistry determine
Production of Human Fab Antibody Fragments from Phage Display Libraries
with the desired binding properties (1 ) (see review in ref. 2 ). Initially, the system was used for mapping antibody epitopes by expression of large libraries (107 –108 ) of random peptides (3 –5 ). However, the approach has proved readily applicable
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