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文献和实验Proteomic Analysis of the Stacked Golgi Complex
Although a great deal is known about the structure and function of most mammalian organelles, comprehensive proteomes are necessary to provide a molecular framework to integrate this information. The Golgi complex is the central organelle
Reconstitution of Golgi Disassembly by Mitotic Xenopus Egg Extract in Semi-Intact MDCK Cells
apparatus was visualized in the cells by expression of green fluorescence protein (GFP)-tagged galactosyltransferase, a marker of trans-Golgi cisternae. Xenopus egg extracts arrested at mitosis or interphase were then prepared and added to the semi-intact
for glycosylating host proteins, are mostly localized to the Golgi apparatus, and their localization is maintained in part by a protein vesicular tethering complex, the conserved oligomeric Golgi (COG) complex. Here we detail a combination of fluorescent lectin
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