- ￥4840
- 赛默飞（Thermo Fisher）
- MA546553
- 2025年06月25日
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文献和实验secondary antibody review -- data from 99 publications
Signaling 18 IgG Alexa Fluor 488 immunocytochemistry 1:2000 18 IgG Alexa Fluor 568 immunocytochemistry 1:2000 19 donkey IgG HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat
, 5% BSA, pH 7.4) for 2 h at room temperature and then probed for CTGF by incubation for 40 min with anti-CTGF antibody at 0.5 µg/ml in blocking buffer. After extensive washing at 37°C, the filters were incubated with either HRP-conjugated donkey anti
minutes in PBS-T (25 ml to 50 ml per wash),shaking.8.During last wash,dilute secondary antibody (goat anti-rabbit HRP at 1:2500 i.e.10μl antibody in 25 ml BSA/PBS-T).Pour off last wash and add antibody.Incubate 60 minutes,room temp with shaking.9.Wash
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