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文献和实验4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody
, 4-hydroxynonenal (4-HNE), is the main product formed from omega 6-PUFA (5 ). 4-HNE, a highly toxic aldehyde product of lipid peroxidation (5 ), is a sensitive marker of oxidative damage and lipid peroxidation and can be evaluated by immunohistochemical staining
v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
, and has characterized pathways for the trafficking of APC both into and out of the nucleus.Antibody specificity is an important factor in the determination of APC localization, and in this chapterwe outline a strategy for the unambiguous detection of APC using
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
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