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- 赛默飞（Thermo Fisher）
- MA545584
- 2025年07月13日
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文献和实验v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
7.6, and 1.37 MNaCl. Store at room temperature. 2. TBS with Tween (TBS-T) (10×): prepare a TBS solution (X1)and add 0.1% (v/v) Tween-20. 3. 5% (w/v) Non-fat dry milk in TBS-T. 4. Primary antibody: APC (Ab-1) antibody from OncogeneResearch.
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
a subset of genomic DNA sequences of type 5'-(NNN )3 -3', whereas the 6ZF library would recognize a subset of genomic sequences of type 5'-(NNN )6 -3'. Given the zinc-finger domains used, both libraries were more likely to recognize (RNN)x -type sequences
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