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文献和实验v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
, and has characterized pathways for the trafficking of APC both into and out of the nucleus.Antibody specificity is an important factor in the determination of APC localization, and in this chapterwe outline a strategy for the unambiguous detection of APC using
antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
is maintainedduring the purification procedure and that a substantialproportion of cellular GSK3 is inhibited (hence, this is subjectto the cell system used and variations in protein purificationthat may disrupt the adenomatous polyposis coli [APC] complex
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