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文献和实验相关专题 IL-33 may be released by damaged epithelial Cell s in response to various noxious agents, and induce the release of several proinflammatory cytokines by cells expressing ST2, such as mast cells, eosinophils and M2
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development
7 ). In this chapter, we describe the procedure for the induction of differentiation into the osteoclast lineage from murine embryonic stem (ES) cells ( 8 ). We utilize co-culture systems with stromal cell lines, that is, bone marrow-derived ST2 cells ( 9 ) or newborn
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