- ￥2716
- 赛默飞（Thermo Fisher）
- MA543540
- 2025年07月16日
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文献和实验Acute Asthma Models to Ovalbumin in the Mouse
, and can be divided in two groups as acute and chronic models. They are characterized by airway hyperresponsiveness (AHR), inflammation, and remodeling. Several experimental procedures have been implemented, the one mostly used being acute asthma models to ovalbumin
-Glycine transfer buffer (e.g. Novex) 100% methanol Blocking solution containing casein (e.g., Pierce) Wash Solution I (for 1L: 1g Ovalbumin (Sigma), 1g Dry milk, 1 ml Tween-20 (Bio-Rad), 100 ml 10X PBS, 900 ml distilled water
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
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