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文献和实验Analysis of CTG Repeats Using DM1 Model Mice
This chapter describes how transgenic mice can be made with human genomic DNA fragments cloned from DM1 patients’ DNA and how the CTG repeat instability is assessed over generations and in different tissues. Construction of cosmid libraries
Cell Recovery from DM1 Transgenic Mouse Tissue to Study (CTG)
Myotonic dystrophy type 1 results from an unstable expanded CTG repeat ((CTG) n ) in the 3′ UTR of the DMPK gene. Transgenic mouse models have been developed to reproduce the (CTG) n instability seen in DM1 patients. These transgenic mice
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
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