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文献和实验Human Placental Alkaline Phosphatase Stain
The following protocol is for detection of human placental alkaline phosphatase (AP) in cultured cells. Human placental AP is heat stable, unlike other AP proteins typically expressed by cells. A few cell types (HeLa cells, for example
Alkaline Phosphatase Fusion Proteins as Tags for Identifying Targets for Placental Ligands
In this chapter, we describe protocols for the generation and characterization of alkaline phosphatase-ligand fusion proteins and their use as tools for the identification of specific ligand-receptor interactions.
Staining protocols for placental explant cultures
). Note that alkaline phosphatase conjugates should be used with caution as this enzyme is expressed by some trophoblast. Resin embed allowfullscreen='true'ding of explant cutures. Examination of semithin resin sections can show the pattern of cell
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