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文献和实验: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
【翻译】单克隆抗体治疗肿瘤发展趋势Nature Reviews Drug Discovery 6, 349-356 (May 2007)
单抗讨论小组报名帖|\|/|单抗讨论小组日常讨论交流贴|\|/|\|/|单抗讨论小组工作交流与工资贴 此话题已收录于“单抗小组讨论话题集锦” By waynepionnet Development trends for monoclonal antibody cancer therapeutics 单克隆抗体治疗肿瘤发展趋势 Janice M. Reichert1 & Viia E. Valge-Archer2 Abstract 摘要
Western Analysis of Transgenic Plants
are transferred to a solid support, which is normally nitrocellulose or polyvinylidene fluoride (PVDF [Millipore, Bedford, MA]). Immunological detection of proteins transferred to a solid support combines ease of handling with accessibility of antibodies
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