- ￥5181
- 赛默飞（Thermo Fisher）
- MA541092
- 2025年07月03日
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文献和实验: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
Clustering Assay for Studying the Interaction of Membrane Proteins with PDZ Domain Proteins
surface. This chapter describes the experimental details of the clustering assay, using the interaction between potassium channels and PSD-95, an abundant PDZ domain protein in neuronal synapses, as a model.
Quantification of Synaptic Structure Formation in Cocultures of Astrocytes and Hippocampal Neurons
astrocytes for 24 h. Neuronal immunocytochemical staining of the presynaptic marker, synaptophysin, and the postsynaptic marker, PSD-95, is used to assess the localization of synaptic proteins into pre and postsynaptic structures. Deconvolved, confocal images
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