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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Fluorescent Immunohistochemistry and In Situ Hybridization Analysis of Pancreas
is always required for different tissues and antigens. Using a low-power antigen retrieval technique, with appropriate dilution of antibodies, we successfully immunostained key antigens in the pancreas such as insulin, PDX-1, glucagon, cytokeratin, and CD31
) 3. BSA 4. DMEM 5. FCS 6. Antibiotic 7. Heparin 8. F12medium 9. Isoflourane 10. Ethonol 11. Dynabeads 12. anti-CD31antibody(BDPharmingen,catalognumber:550274) 13. M199 14. gelatin 二、设备 1. 离心机 2. 无菌培养橱
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