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文献和实验CD80 分子 CD80 常用单克隆抗体或代号: B7- 1,BB1 主要表达细胞: Ba,Mac,TStr [B] 分子质量(kDa)和结构: gp60(IgSF) 功 能: 活化B细胞抗原,CD28、CTLA- 4配体,提供T细胞共刺激信号 CD80 Aka B7-1, BB1 Ligand for T cell marker CD28 or CTLA4 (CD152
Non-Replicating Recombinant Vaccinia Virus Expressing CD80 to Enhance T-Cell Stimulation
The following method describes the generation of a recombinant vaccinia virus expressing a costimulatory molecule (human CD80 or B7.1). The procedure first requires the cloning, by classical methods not described here, of the gene
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
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