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文献和实验Use of Gene Targeting to Study Recombination in Mammalian Cell DNA Repair Mutants
(“knockout”) mutants in the repair/recombination gene ERCC1 . Using this approach, we have constructed pairs of isogenic ERCC1 -proficient and -deficient (null) CHO cell lines and used them as recipients for gene targeting assays in which a hemizygous mutant
confirmation. A. Protein microarray hybridization with 8F1. The OriGene overexpression protein microarray chip was immunostained with the most commonly used 8F1 monoclonal anti-ERCC1 antibody. The positive reactive proteins are highlighted with red arrows
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
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