- ￥6161
- 赛默飞（Thermo Fisher）
- MA537915
- 2025年07月12日
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文献和实验Primary Neuron Viability Assay
with 1 ml of PBS and the cellular fluorescence remaining on the plate was determined with a fluorescent plate reader (CytoFluor II, Persceptive Biosystems, MA) with excitation at 485 nm and emission at 530 nm. The resulting data were analyzed using GraphPad
). Primary antibody Horseradish peroxidase-conjugated secondary antibody Wash Solution II (for 1L: 1 ml Tween-20, 100 ml 10X PBS, 900 ml distilled water) Luminol and oxidizing reagents (e.g., Pierce or NEN) 2. Methods
Whatman 3MM or equivalent, cut to same size as gel Wetting Solution 100% Methanol Anode Buffer I 300 mM Tris, 20% methanol, pH 10.4 Anode Buffer II 25 mM Tris, 20% methanol, pH 10.4 Cathode Buffer 25 mM Tris, 20% methanol, 40 mM 6-aminohexanoic acid, pH
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