- ￥6161
- 赛默飞（Thermo Fisher）
- MA537862
- 2025年07月15日
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文献和实验PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
affinity) and the other is an IP kinase reaction. Substrate/Antibody, Lysate Interaction: 1. For GST c-Jun substrate interaction the quantity of protein on the beads is variable with the batch made (make fresh at least once a week). Generally
Purification of Myosin Light Chain Kinase
to ~10 mMHO with Buffer F.Load the above solution on to a calmoudlinsepharose column (20 ml Bed volume pre-equilibrated with Buffer F; max.0.6 mg kinase/ml CaMsepharose). Collect unbound pool in 250 ml beaker (pump = 20x).When completely loaded,gently
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