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文献和实验提示我模型中的通路究竟涉及哪个亚型,而我又没有那么多经费一个一个亚型去试,故我想能不能就检测总的PKC激活水平,毕竟有亚型被激活,那总的活化PKC水平也应该会升高啊!虽然敏感性和特异性肯定没有单独检测一个亚型的高。 还想请教:Phospho-PKC (pan) (gamma Thr514) Antibody 和Phospho-PKC (pan) (βII Ser660) Antibody 这两个抗体的针对的亚型有什么不同?既然都是pan,那是否说都是针对所有的亚型,只是磷酸化的残基不同
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
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