- ￥5109
- 赛默飞（Thermo Fisher）
- MA536193
- 2025年07月16日
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文献和实验Phosphorylation of Amyloid Precursor Protein (APP) Family Proteins
including fly (4 ), nematode (5 ), and fish (6 ). In mammals, two APP-like genes, amyloid precursor-like protein 1 (APLP1) and 2 (APLP2), have been isolated (7 ,8 ). The amino acid sequences of these APP family proteins are highly conserved, especially
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
Western Analysis of Transgenic Plants
are transferred to a solid support, which is normally nitrocellulose or polyvinylidene fluoride (PVDF [Millipore, Bedford, MA]). Immunological detection of proteins transferred to a solid support combines ease of handling with accessibility of antibodies
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