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文献和实验v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
electrophoresis equipment. 2. Transfer buffer: 25 mM Tris, 192 mM glycine, and 10% (v/v)methanol, pH 8.3. 3. Gel blot paper. 4. Polyvinylidene difluoride (PVDF) membrane (Millipore,Billerica, MA). 5. Methanol. 6. Tris-buffered saline (TBS): 10 mM
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Strategies to Optimize Protein Expression in E. coli
, purification, and characterization of the maleylacetate reductase from sphingobium chlorophenolicum strain ATCC 53874. Curr. Microbiol. 58:599‐603. Chen, X., Tong, X.T., Xie, Y.H
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