- ￥4787
- 赛默飞（Thermo Fisher）
- MA535997
- 2025年07月15日
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文献和实验Basic Protocol 3: Secondary Screening and Analysis of Primary Hit Compounds of Proteasome‐Associated USP14 Materials AMC amine (Sigma
Primary Neuron Viability Assay
antibody (N-4142; Sigma, St. Louis, MO) demonstrated that approximately 80-90% of the cells present were neurons. Neuronal viability was assessed with the fluorescent viability dye, calcein-AM (Molecular Probes, Eugene, OR. Briefly, neuronal cultures
and then centrifuge at 14,000 RPM for 3 minutes. Save 20µl of the elutes as (Flag-Input). Next, take the remaining ~80µl of elute to clean tubes containing 40µl of EZVIEW™ Red anti-Flag M2 affinity gel beads (Sigma™) which have been pre-treated with a wash in 250µl
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