- ￥4824
- 赛默飞（Thermo Fisher）
- MA535315
- 2025年07月15日
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文献和实验Preparation and Analysis of Drosha
Drosha is a member of the ribonuclease (RNase) III family. Like other RNase III proteins, Drosha is a double-stranded RNA (dsRNA)-specific endonuclease that introduces staggered cuts on each strand of the RNA helix (1 ,2 ). RNase III
Primary MicroRNA Processing Assay Reconstituted Using Recombinant Drosha and DGCR8
In animals, the Microprocessor complex cleaves primary transcripts of microRNAs (pri-miRNAs) to produce precursor microRNAs in the nucleus. The core components of Microprocessor include the Drosha ribonuclease and its RNA-binding partner
:501–505), and target recognition (Jin et al., Methods Mol Biol 936:117–127). Here we describe the use of a dual-luciferase reporter system to evaluate the enzymatic activity of a key enzyme involved in RNA maturation—DROSHA. This dual
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