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文献和实验: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
Lymphatic Endothelial Cells: Establishment of Primaries and Characterization of Established Lines
lymphatic endothelial cells requires a two-step process: mechanical and enzymatic digestion of human foreskins and cell sorting by fluorescence-activated cell sorting of CD31+ /podoplanin+ /CD45− cells. Characterization of these cells requires an assessment
Lymphatic Invasion as a Prognostic Biomarker in Primary Cutaneous Melanoma
). With the discovery of lymphatic endothelial cell markers, such as podoplanin and LYVE-1, lymphatic vessels can be reliably detected in formalin-fixed paraffin-embedded (FFPE) tissues. There is a now consensus that lymphatic invasion detected by immunohistochemical
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